Journal: Journal of Ovarian Research

Article Title: MSCs–derived EVs protect against chemotherapy-induced ovarian toxicity: role of PI3K/AKT/mTOR axis

doi: 10.1186/s13048-024-01545-7

Figure Lengend Snippet: Effect of MSCs-EVs, rapamycin, and quercetin on ( A ) phosphorylation of mTOR, PI3K, and AKT as detected by western blot analysis, ( B - D ) Intensity of immunoreactivity of selected proteins as quantified by densitometry. Results are expressed as mean ± SEM. **significant vs. Control group at p<0.01, **** at p<0.0001, # significant vs. OF group at p<0.05, ## at p<0.01, ### at p<0.001, #### at p<0.0001, & significant vs. OF + Rapamycin group at p<0.05

Article Snippet: Next, the blots were incubated with the appropriate primary antibodies for an entire night at 4 °C, PTEN (E-AB-63495, Elabscience, USA), FOXO3 (NBP2-16521, Novus Biologicals USA), mTOR (sc-517464, Santa Cruz Biotechnology, USA), Phospho-mTOR (sc-293133, Santa Cruz Biotechnology, USA), PI3K (E-AB-64202, Elabscience, USA), Phospho-PI3K (E-AB-20966, Elabscience, USA), AKT (E-AB-15441, Elabscience, USA), Phospho-AKT (E-AB-20804, Elabscience, USA), β-actin (E-AB-20031, Elabscience, USA).

Techniques: Phospho-proteomics, Western Blot, Control

Journal: Journal of Functional Foods

Article Title: 3-Fucosyllactose alleviates DSS-induced mouse colitis through modulation of the PI3K-Akt signaling pathway: An integrated multi-omics analysis

doi: 10.1016/j.jff.2025.106789

Figure Lengend Snippet: Fig. 2. 3-FL supplementation induces changes in mouse colon transcriptome, primarily affecting genes in the PI3K-Akt signaling pathway. (A) Volcano plot of DEGs between 3-FL and DSS group. (B) Heatmap of the top 50 DEGs in 3-FL and DSS. (C) KEGG Pathway Enrichment Analysis of Upregulated DEGs. (D)KEGG Pathway Enrichment Analysis of Downregulated DEGs. (E) Spearman correlation heatmap between the DEGs and phenotypic indicators.

Article Snippet: AKT, Phospho-AKT (Ser473,p-Akt), PI3K p85, and Phospho-PI3 Kinase p85 (Tyr458,p-PI3K) antibodies were from CST Corporation (Boston, USA) and Servicebio Co., Ltd. (Wuhan, China).

Techniques:

Journal: Journal of Functional Foods

Article Title: 3-Fucosyllactose alleviates DSS-induced mouse colitis through modulation of the PI3K-Akt signaling pathway: An integrated multi-omics analysis

doi: 10.1016/j.jff.2025.106789

Figure Lengend Snippet: Fig. 6. Multi-omics integration analysis of gut microbiota, microbial metabolites and DEGs associated with the PI3K-Akt signaling pathway. (A) Spearman correlation heatmap between the DEGs and differential metabolites. (B) Sankey plot illustrating the interactions between gut microbiota, microbial metabolites, and DEGs associated with the PI3K-Akt signaling pathway. Compare with indicated group, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Article Snippet: AKT, Phospho-AKT (Ser473,p-Akt), PI3K p85, and Phospho-PI3 Kinase p85 (Tyr458,p-PI3K) antibodies were from CST Corporation (Boston, USA) and Servicebio Co., Ltd. (Wuhan, China).

Techniques: Biomarker Discovery

Journal: Journal of Functional Foods

Article Title: 3-Fucosyllactose alleviates DSS-induced mouse colitis through modulation of the PI3K-Akt signaling pathway: An integrated multi-omics analysis

doi: 10.1016/j.jff.2025.106789

Figure Lengend Snippet: Fig. 7. Western blot analysis demonstrated that 3-FL exerts an inhibitory effect on the PI3K-Akt signaling pathway. (A)The levels of PI3K, p-PI3K, Akt, and p- Akt in colon tissues were measured by Western blot. (B–C) Quantitative analysis of (A).

Article Snippet: AKT, Phospho-AKT (Ser473,p-Akt), PI3K p85, and Phospho-PI3 Kinase p85 (Tyr458,p-PI3K) antibodies were from CST Corporation (Boston, USA) and Servicebio Co., Ltd. (Wuhan, China).

Techniques: Western Blot

Journal: Molecular Medicine Reports

Article Title: SDF-1α/CXCR4 signaling promotes capillary tube formation of human retinal vascular endothelial cells by activating ERK1/2 and PI3K pathways in vitro

doi: 10.3892/mmr.2022.12821

Figure Lengend Snippet: Effect of SDF-1α/CXCR4 signaling on the protein expression of ERK1/2 and PI3K pathway. (A) Representative results of ERK1/2, p-ERK1/2, PI3K and p-PI3K expression of HRECs were determined using western blotting. SDF-1α/CXCR4 signaling significantly increased the protein expression of ERK1/2 and PI3K pathway. (B) Statistical analysis of ratios of p-ERK1/2 and p-PI3K to GAPDH protein. (C) Statistical analysis of ratios of p-ERK1/2/total ERK1/2 and p-PI3K/total PI3K. All values represent mean ± standard error of the mean (n=3). *P<0.05 vs. control. SDF, stromal cell-derived factor; CXCR, chemokine (C-X-C motif) receptor; p-phosphorylated; HRECs, human retinal endothelial cells.

Article Snippet: Rabbit anti-Erk 1,2 monoclonal antibody (cat. no. orb178404; Clone B20-U; 1:5,000), Rabbit anti-phosphorylated (p-) ERK 1,2 monoclonal antibody (cat. no. orb178405; Clone G15-B; 1:5,000), rabbit anti-PI3K P85 (phospho-Tyr467) polyclonal antibody (cat. no. orb14998, 1:5,000) and rabbit anti PI3K polyclonal antibody (cat. no. orb1089274, 1:1,000) were purchased from Biorbyt Ltd. Human retinal vascular endothelial cells (HRECs; cat. no. YS0884) were purchased from Yaji Biological Technology Co., Ltd.

Techniques: Expressing, Western Blot, Control, Derivative Assay